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Coding

Part:BBa_K1331000:Design

Designed by: Jiayi Dong   Group: iGEM14_Nankai   (2014-09-25)


RhlA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 343
    Illegal SpeI site found at 258
    Illegal PstI site found at 303
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 258
    Illegal PstI site found at 303
    Illegal NotI site found at 324
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 252
    Illegal XhoI site found at 331
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 343
    Illegal SpeI site found at 258
    Illegal PstI site found at 303
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 343
    Illegal SpeI site found at 258
    Illegal PstI site found at 303
    Illegal NgoMIV site found at 669
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

we did a mutation to fit the Igem requirment


Source

From a key lab of Nankai University

References